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Image Search Results
Journal: Biomolecules & Therapeutics
Article Title: Enhancement of Radiosensitivity by DNA Hypomethylating Drugs through Apoptosis and Autophagy in Human Sarcoma Cells
doi: 10.4062/biomolther.2021.174
Figure Lengend Snippet: Low-dose 5-aza-dC and SGI-110 treatments can induce demethylation in human sarcoma cell lines. Western blots show the protein expression levels of DNMT1 (180 kD) in human sarcoma cell lines (SK-LMS-1, SK-UT-1, SK-UT-1B, and SK-ES-1) treated with different doses (0.1/0.5/1 μM) of 5-aza-dC or SGI-110. The blots were probed by using anti-DNMT1 (upper panel) and anti-β-actin antibodies.
Article Snippet:
Techniques: Western Blot, Expressing
Journal: Biomolecules & Therapeutics
Article Title: Enhancement of Radiosensitivity by DNA Hypomethylating Drugs through Apoptosis and Autophagy in Human Sarcoma Cells
doi: 10.4062/biomolther.2021.174
Figure Lengend Snippet: The combination of 5-aza-dC or SGI-110 and IR contributes to inducing apoptosis in sarcoma cell lines. (A) The levels of apoptosis were measured by using Annexin V and 7-amino-actinomycin, after which they were analyzed by using a FACS flow cytometer. The levels of apoptosis in SK-LMS-1 and SK-UT-1 cells that were treated with 5-aza-dC (0.1 μM) or SGI-110 (0.1 μM) either alone or with irradiation (2 and 4 Gy) are expressed as percentages of the total cell population at both the early and late stages of apoptosis. (B) The activities of caspases 3 and 7 were determined by using the Caspase-Glo assay and are represented as percentages of SK-LMS-1 and SK-UT-1 cells that were treated with 5-aza-dC or SGI-110 either alone or with irradiation (2 and 4 Gy), compared with untreated cells. The graph represents data (mean ± SD) from three independent experiments. No significant (NS) was not indicated but statistically significant p value was indicated in the graph. * p <0.05, ** p <0.01, *** p <0.001. (C) Western blot analysis of the expression levels of apoptosis-associated proteins (cleaved caspase 3, cleaved caspase 9, and cleaved PARP1) in sarcoma cell lines following treatment with 5-aza-dC, SGI-110, or IR alone and in combination.
Article Snippet:
Techniques: Flow Cytometry, Irradiation, Caspase-Glo Assay, Western Blot, Expressing